Nano+2010

__Nano Group Student: Lauren

Monday, June 21, 2010 and Tuesday, June 22, 2010__ - Basic lab techniques, notebook - How to prepare an APTES monolayer - How to prepare solutions - How to perform depositions - Training on AFM (Atomic Force Microscopy) imaging in air and fluid - Research area background (viewed powerpoint presentations) - Marya Lieberman group meeting - Toured research labs

__Wednesday, June 23, 2010__ - [] - [] - __Basic laboratory notebook guidelines:__ o Make in permanent medium. o Use consecutive pages. o Date entries. o Identify subject matter. o Include sketches, diagrams, etc. o Explain sketches, etc. o Photos, drawing, etc., should be identified and permanently attached. o Avoid erasures. o Don't change entries; make new entry. o Periodically have someone look over entries and witness same by applying signature and date. - Discuss alpha particle experiment - Image mica and silicon at JHS - Order supplies (goggles, labcoat, microscopes), - Make spreadsheet to outline future experiments - Get sticky tape for SEM <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- General Lab safety training class, MSDS (material safety data sheets) o Lisa Boagner <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Make sure that computer has software needed

__Thursday, June 24, 2010 and Friday, June 25, 2010__ <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Nanosurf AFM Scanning o Three samples: § Alpha particles on Gold (Au) · 15 Minutes · 1 Hour · 2 Hours § Mica § Aluminum (Al) <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Assisted Sharon Brandt o Scanning Electron Microscope § Sample preparations § Computer troubleshooting <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Summary o A total of 12 images were obtained on the Atomic Force Microscope. All images were saved for further processing with Valerie Goss. Various sized images were obtained: 1um, 9 um, 19 um, and 72 um. Four regions were scanned. Initial analysis shows problems with tip interfacing with the surface, tip artifacts, and fast-scanning errors. <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Future o More samples will be obtained o Slower scan speeds will be employed o Focus on larger scan sizes (20 um and higher) <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Images by Lauren and Valerie

__ Monday, June 28, 2010 __ <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Took notes on basis of DNA origami <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- With Becky Quardokus o Learned how to work the Scanning Tunneling Microscope (STM) <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Took images of gold sample

__ Tuesday, June 29, 2010 __ <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Looked at previously taken images/analyzed them <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Marya Lieberman group meeting __ Wednesday, June 30, 2010 __ <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Took images (4) on the Atomic Force Microscope (AFM) <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Parameters are on pg 25 of Notes/Journals lab book

__ Thursday, July 1, 2010 __ <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Went over how to make the TAE/Mg+2 Buffer for DNA origami liquid imagine on the AFM <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Carrie Miller practice for talk on PhD

__ Friday, July 2, 2010 __ <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Prepared the solution of TAE/Mg+2 Buffer o Didn’t autoclave <span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Took images of DNA origami under liquid (old DNA and old buffer solution)

<span style="mso-ascii-font-family: Calibri; mso-bidi-font-family: Calibri; mso-fareast-font-family: Calibri; mso-hansi-font-family: Calibri;">- Images by Lauren and Valerie 60 minute alpha particle bombardment (shielded part)